Distilled water was placed before each trial to ensure the accuracy of results. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength. Regardless, my hypothesis is accepted because of the evidence from my results paired with already given knowledge about Spectrophotometer essay glucose from previous studies done by others.
I had a sample from immediately before a meal, 30 minutes after, one hour later, an hour and a half later, and then finally two hours after the meal. Four known concentrations were prepared; Spectrophotometer essay. Determining the concentration of the unknown sample was done by drawing a graph of the concentration against the absorbance and was found to Spectrophotometer essay Introduction Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution.
Both blood types peaked in absorbency one hour after the meal. The experiment went as I expected it to. Then only a specific wavelength of light shines through the spectrophotometer and interacts with the solute.
Get Full Spectrophotometer essay Get access to this section to get all help you need with your essay and educational issues. Transmittance is the amount of light that gets through the sample. In this lab we will use a few different solutions in the spectrophotometer to get a basic feel on how it works.
Spectrophotometry is one of the most useful methods of quantitative analysis in various fields such as chemistry, physics, biochemistry, material and chemical engineering and clinical applications. By doing so I found that for diabetics the maximum blood glucose increased more quickly than non-diabetics, which increased and decreased at a steady rate.
The first step in determining the absorbance for the blood samples for comparison was to measure the amount of light absorbed by each solution. Some of these negative effects include heart disease, high blood pressure, kidney problems, and nerve damage Effects of Diabetes, The light that continues past the solute hits the phototube.
A spectrophotometer is an instrument that measures the amount of photons the intensity of light absorbed after it passes through sample solution. Learning This lab will teach me how to use a spectrophotometer.
There should also be more studies on specific food types to see if there are negative of positive effects on diabetics. With this information I was able to determine that the damaging effects of glucose molecules would be higher in diabetics than in non-diabetics. The use of the spectrophotometer is to measure the concentration of solute.
This shows how much light got trapped in the solute. With the spectrophotometer, the amount of a known chemical substance concentrations can also be determined by measuring the intensity of light detected.
We will then graph our results and compare them with the class values of the three unknown Methylene blue samples. From this information, one can conclude that the damaging effects would be higher in diabetics. Diabetics effects too many people in the world to not have more abstract studies done on it.
I did this by measuring the amount of light absorbed by the solutions through a spectrophotometer. After recording the absorbance percentages of all my blood samples I created a line graph in order to physically see the trends and differences between the blood glucose levels of the diabetic and non-diabetic blood.
We will record the absorbance as well as do calculations using mean and standard deviation. The diabetic blood absorbed much more quickly but decreased relatively as steady as the non-diabetic.
I used a spectrophotometer to do so. With the experiment I hypothesized that the diabetic blood would have a higher absorbance reading than the non-diabetic. A standard of potassium permanganate solution was prepared and computed to be 0.
Experiment 9 focuses on determining the different concentrations of potassium permanganate solutions by finding its absorbance through the use of spectrophotometer. At the end of the lab the actual concentrations of each unknown will Spectrophotometer essay shared.
The food eaten during the meal may vary my results slightly, since some foods can raise and lower glucose levels more than others. This lab will teach me how to use a spectrophotometer. Further studies should be done at different times of the day to determine how correlated certain meals are with glucose levels.
Absorbance is the opposite of transmittance and the reciprocal of it. We will then compare how accurate and precise our results are with the actual.Determining Concentrations with Spectrophotometer. Print Reference this To learn how to make a spectrophotometer accurately and interpret the data recorded to construct a graph and obtain a standard curve using excel.
If you are the original writer of this essay and no longer wish to have the essay published on the UK Essays website. Using Spectrophotometry to Measure “Blood Glucose” Essay Sample. In the experiment, I tested the blood glucose level of diabetic and non-diabetic people every 30 minutes starting from right before a meal was eaten up until two hours.
Spectrophotometer is a kind of photometer which is used in molecular biology. It is used for determining the amount of substance in solution. It is used for determining the amount of substance in solution.
This lab will teach me how to use a spectrophotometer. The use of the spectrophotometer is to measure the concentration of solute. The solute being measured must be colored and is determined based on the adsorption of light photons on a wavelength.
The spectrophotometer uses a beam of light that strikes the diffraction grating that [ ]. Read this essay on Spectrophotometry. Come browse our large digital warehouse of free sample essays. Get the knowledge you need in order to pass your classes and more. Only at killarney10mile.com".
Spectrophotometry is one of the most useful methods of quantitative analysis in various fields such as chemistry, physics, biochemistry, material and chemical engineering and clinical applications.
A spectrophotometer is an instrument that measures the amount of photons (the intensity of light) absorbed after it passes through sample solution.Download